Determination of Plasmid DNA Purity Ratio in Human Gene Therapy Products Using Slope Spectroscopy®
Estimation of DNA purity in the presence of protein for gene therapy plasmid material is possible by exploiting the 260/280 UV absorbance ratio. This technique, however, has significant challenges when accurate quantification of DNA purity is required. The SoloVPE and its Slope Spectroscopy technique offer a new method to overcome these challenges, and to accurately measure DNA purity ratios in accordance with the Beer-Lambert law.
This application note demonstrates the SoloVPE’s ability to accurately measure the R value within plasmids. The Slope Spectroscopy technique addresses users’ challenges with sample volume and dilution and inconsistent wavelength readings, enabling the instrument to provide reliable data based on specified parameters within the software. Despite all the challenges, the SoloVPE enables users to quickly and precisely measure absorbance readings at variable pathlengths. Read it here!